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Uncemented primary total hip arthroplasties were inserted in the femoral heads of rabbits. The rabbits were sacrificed at 4 and 12 weeks after surgery and histochemical, biochemical, and molecular biological techniques were used to examine the effects of the prosthesis on osteoclasts and osteoblasts. Biochemical data showed that the total resorption surface at 4 weeks was reduced to 50% of normal, and the ion-product surface was also reduced. Thus, the osteoclasts appeared to be inhibited by the prosthesis. The resorption area and number of osteoclasts was significantly reduced at 12 weeks compared with 4 weeks. Histochemistry showed the presence of osteoblasts in the interface between the bone and cement. No chemical or calcium binding was detected, although there was cell proliferation in the cement. A large number of tartrate-resistant acid phosphatase positive tartrate-resistant acid phosphatase (TRAP) cells were found in the cement at 4 weeks. The TRAP activity was significantly reduced by the end of the 12th week. These data indicate that the resorption of the bone-cement interface is inhibited by uncemented total hip arthroplasties. This is likely due to the presence of a strong inhibition factor in the cement. The changes seen in osteoblasts, however, were less pronounced. This difference suggests that the osteoblasts are affected more by mechanical stress than the osteoclasts. Our results also suggest that the inhibition of the osteoclasts is much more pronounced at 4 weeks than at 12 weeks.Q:
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