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We generated a library of 9927 genes from the yeast genome with high confidence open reading frames, using the criteria suggested by Wapinski et al \[[@B1]\]. Each of these genes was targeted for homologous recombination using the S. pombe knockout collection \[[@B2]\]. The library will be of value for researchers seeking to analyze gene function and for researchers attempting to unravel biological processes in fission yeast.

To facilitate the identification of these mutant strains, we constructed a single S. pombe strain in which all genes had been knocked out \[[@B3]\]. We also generated a series of antiserum against recombinant fission yeast proteins to make it easier to identify the cells with a knock-out and to monitor protein expression. These antisera were raised against linear peptides, suitable for immunofluorescence microscopy.

Materials and methods

Library genes were heterologously expressed in the single S. pombe KO strain. The expressed proteins were purified and used to generate antisera (Fig [1A](#F1){ref-type=”fig”}). Antisera were used to immunoprecipitate tagged fission yeast proteins using streptavidin beads, and proteins were separated by two-dimensional gel electrophoresis (Fig. [1B](#F1){ref-type=”fig”}).

![**Antibodies raise against fission yeast proteins.** A. Antibody production. m, mouse. p, rabbit. B. 2D-gel. These proteins were separated by 2D-gel electrophoresis and stained with Coomassie blue. Numbers indicate sizes of the spots in kDa.](1753-6561-1-S1-S40-1){#F1}


We have generated a comprehensive antibody library of proteins from the fission yeast genome, which consists of hundreds of antibodies against 9927 genes. We find that one antibody is raised against every protein in the yeast genome. Each of these genes was targeted


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